ultra-sensitive multi-array assay  (Meso Scale Diagnostics LLC)

Journal: Antiviral Research

Article Title: Intranasal treatment with a novel immunomodulator mediates innate immune protection against lethal pneumonia virus of mice

doi: 10.1016/j.antiviral.2016.10.008

Figure Lengend Snippet: Protein expression levels of chemokine and cytokine genes in lung homogenates at 6, 24, 96 and 144 h p.t. Mice were given intranasal treatment of PBS or P-I-P and the multi-lobed lungs of 5 mice per group were collected separately for each individual animal at the time points indicated. Protein expression was assayed by electrochemiluminescence-based multiplex ELISAs. Panels A to H represent protein expression data in pg/mL for CCL2, CXCL1, TNF-α, IL-1β, IL-6, total IL-12 (p70), IL-10, and IFN-γ, respectively. Data are shown as individual values representing single animals as well as median with interquartile range of five biological replicates. *p < 0.05, **p < 0.01.

Article Snippet: The MSD Multi-Spot V-PLEX assay for the pro-inflammatory panel 1 (TNF-α, IL-1β, IL-6, IL-12, IL-10 and CXCL1) and the MSD Multi-Array Mouse Cytokine Ultra-sensitive Assay for CCL2 were used to quantify cytokine, type II IFN, and chemokine levels in the lungs (Meso Scale Discovery, Rockville, MD, USA).

Techniques: Expressing, Electrochemiluminescence, Multiplex Assay

Journal: Antiviral Research

Article Title: Intranasal treatment with a novel immunomodulator mediates innate immune protection against lethal pneumonia virus of mice

doi: 10.1016/j.antiviral.2016.10.008

Figure Lengend Snippet: Protein expression levels of chemokine and cytokine genes of P-I-P pre-treated PVM-infected Balb/c mice. Mice were given an intranasal treatment of PBS or P-I-P 24 h before challenge with 3000 pfu PVM. The multi-lobed lungs of 5 mice per group were collected separately for each individual animal on days 0 (prior to infection), 3 and 5 p.i. Protein expression was assayed by electrochemiluminescence-based multiplex ELISAs. Panels A to H represent protein expression data in pg/mL for CCL2, CXCL1, TNF-α, IL-1β, IL-6, total IL-12 (p70), IL-10, and IFN-γ, respectively. Data are shown as individual values representing single animals as well as median with interquartile range of five biological replicates. *p < 0.05, **p < 0.01.

Article Snippet: The MSD Multi-Spot V-PLEX assay for the pro-inflammatory panel 1 (TNF-α, IL-1β, IL-6, IL-12, IL-10 and CXCL1) and the MSD Multi-Array Mouse Cytokine Ultra-sensitive Assay for CCL2 were used to quantify cytokine, type II IFN, and chemokine levels in the lungs (Meso Scale Discovery, Rockville, MD, USA).

Techniques: Expressing, Infection, Electrochemiluminescence, Multiplex Assay